DeltaTime

The DeltaTime TCSPC lifetime plug-in

TCSPC Lifetime Kit

Fast, flexible and affordable plug-in TCSPC lifetime system for HORIBA Steady State Instruments, outperforming any other multifunctional fluorescence system on the market, integrating accessories with the widest array of picosecond light sources

The DeltaTime time correlated single photon counting (TCSPC) lifetime plug-in offers acquisition speed, flexibility, and affordability unavailable in any other multifunctioanl fluorescence solution on the market. DeltaTime seamlessly integrates monochromators, polarizers, and other accessories with the widest array of sources (LEDs, laser diodes, supercontinuum lasers) and detectors (including NIR), providing lifetime coverage from 25ps to 1sec over wavelengths spanning the UV to NIR.

The culmination of over 40 years of lifetime experience, DeltaTime highlights include: the fastest sources (up to 100 MHz), the widest lifetime ranges (ps to sec), virtually unlimited configurability and advanced lifetime analysis software. DeltaTime is part of our Delta series - truly the next generation of fluorescence lifetime systems.

Segment: Scientific
Division: Fluorescence
Manufacturing Company: HORIBA Scientific
  • Fast…lifetime acquisition times from one millisecond
  • Sensitive…uses single-photon counting detection
  • Accurate…crystal locked timing circuits never require recalibration
  • Wide range…resolves lifetimes from 25ps to 1 second
  • Modular…easily reconfigured as measurement requirements evolve
  • Compact…desktop dimensions
  • Convenient…single USB 2.0 connection to PC
 DeltaPro-DDDeltaPro-NL
Minimum lifetime25 ps with laser-diode source30 ps with laser-diode source
Shortest acquisition time1 millisecond*100 milliseconds*
Diode controllerDeltaDiode and SpectraLEDNanoLED and SpectraLED
Repetition rates 
  • 10 kHz–100 MHz with DeltaDiode*
  • 0.1 Hz–10 kHz with SpectraLED
 
 
  • 10 kHz–1 MHz with NanoLED
  • 0.1 Hz–10 kHz with SpectraLED
 
Prompt FWHM<200 ps FWHM with PPD and laser diode
Dead time10 ns
Time ranges10 ns – 11 s100 ns–11 s
Wavelength selectionInterchangeable filters (filters purchased separately from HORIBA or others)
Detector response250–650 nm standard; 250–850 nm and 300–900 nm optional
PC interfaceUSB 2.0. PC not included. Requires Windows® XP or Windows®  7, 32/64-bit English language ver.
System footprint75 cm * 45 cm nominal excluding PC (depending on options)
Fluorescence Anisotropy Studies
Fluorescence Anisotropy Studies
Polarized light striking a fluorescent molecule results in polarized fluorescence. This polarized emission gradually returns to unpolarized fluorescence depending on rotational diffusion and other factors. Anisotropy is directly related to the polarization, and is the ratio of the polarized light component to the total light intensity.
Dye‐protein binding monitored in a microliter volume using timeresolved fluorescence
Dye‐protein binding monitored in a microliter volume using timeresolved fluorescence
The potential health benefits stemming from the antioxidant activity of curcumin, commonly found in turmeric (Curcuma longa L), has attracted the interest of several research groups.
Plasmon enhancement of protein fluorescence by silver nanostructures
Plasmon enhancement of protein fluorescence by silver nanostructures
The use of metal surfaces in conjunction with fluorescence molecules employing a plasmon effect, sometimes referred to as metal enhanced fluorescence, can be advantageous because of the possible enhancement of photophysical properties. For example, the emission intensity of the fluorophore, can be improved.
Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding
Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding
Rapid mixing accessories to perform stopped flow measurements have found application in characterizing interactions and reactions occurring in solution. Reactants are expelled from syringes, mixed and injected into a flowcell.
Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles
Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles
Upconverting lanthanide-based nanomaterials exhibit a unique fluorescence anti-Stokes shift, which enables them to convert NIR wavelength excitation into visible shorter wavelength emissions (NIR to UV-Vis).
Characterizing Lanthanides in Glasses for Optical Applications
Characterizing Lanthanides in Glasses for Optical Applications
Glasses are essential materials with a multitude of uses and many forms. In the area of optoelectronics there is an interest to modify the glass composition to favor the incorporation of lanthanide elements.
Upconversion of Lanthanide-containing glasses using DD‐980L excitation
Upconversion of Lanthanide-containing glasses using DD‐980L excitation
The phenomenon of upconversion is an optical process that takes in lower energy (longer wavelength) photons and emits higher energy (shorter wavelength) photons.
Measurement of carrier lifetime in perovskite for solar cell applications
Measurement of carrier lifetime in perovskite for solar cell applications
Hybrid perovskite photovoltaics (PV) show promise because of their good efficiencies, which can be around 20%. Along with their PV characteristics, perovskite materials exhibit a high degree of radiative recombination.
Time‐resolved luminescence of security inks from the UV to NIR
Time‐resolved luminescence of security inks from the UV to NIR
The use of security features, such as luminescent inks, has increased significantly in an attempt to prevent fraud and counterfeiting of materials and goods.
Time‐resolved Fluorescence for Monitoring Food Composition
Time‐resolved Fluorescence for Monitoring Food Composition
The use of time‐resolved fluorescence has expanded as the relative cost of instrumentation has decreased in recent years. One area where this is especially true is in the food industry, where time‐resolved fluorescence has been applied in the characterization of food stuffs as well as aspects related to food safety and degradation.
Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques
Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques
Light incident on a leaf can be absorbed by chlorophyll to commence the photosynthetic cycle. Excess energy can be liberated as heat or by emission of fluorescence and this can be used to assess the efficiency of the photosynthetic process.
Visualizing dental caries using fluorescence lifetime microscopy
Visualizing dental caries using fluorescence lifetime microscopy
Teeth are naturally fluorescent and changes in their composition, caused by decay for example, affect their fluorescence behavior.
The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal
The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal
The study of singlet oxygen (1O2) is of interest, principally, as it is a highly reactive species. It can be produced by photosensitisation, usually of a molecule such as a dye or porphyrin. Thus, by the appropriate selection of sensitiser, the presence of oxygen and light, 1O2 can be selectively generated. From a biological aspect it has the ability to damage and destroy cells, which has lead to interest in its use as an anticancer agent in photodynamic therapy (PDT).
Visualizing local viscosity using fluorescence lifetime microscopy
Visualizing local viscosity using fluorescence lifetime microscopy
The use of fluorescent molecules, known as molecular rotors, is advantageous in estimating the local (nanoscale) viscosity in microheterogeneous systems, since it just requires the measurement of their fluorescence lifetime.
Effect of temperature on HSA structure inferred using timeresolved room-temperature phosphorescence
Effect of temperature on HSA structure inferred using timeresolved room-temperature phosphorescence
To access intrinsic amino acids, such as tryptophan, as probes, the UV excitation wavelengths for pulsed phosphorescence measurements have long been the preserve of low-repetition-rate gas-filled lamps or larger laser systems. Recent developments have enabled the use of interchangeable semiconductor diodes...
Investigating photocleavage using time‐resolved emission spectra
Investigating photocleavage using time‐resolved emission spectra
The choice of protecting group is of crucial importance in the success of many steps in organic synthesis and the manipulation of polyfunctional molecules, since they can prevent the formation of undesired side products and reactions.
Elucidating Local Viscosity Using Fluorescence Lifetime Measurements
Elucidating Local Viscosity Using Fluorescence Lifetime Measurements
Certain fluorescent molecules, known as molecular rotors, can be employed to estimate the local (nanoscale) viscosity in microheterogeneous systems by measurement of their fluorescence lifetime. This can be advantageous over the usual fluorescence anisotropy method, as the measurement is simpler and faster to perform. This is demonstrated using the HORIBA Scientific TemPro fluorescence lifetime system to monitor the gelation of silica produced using the sol‐gel technique.
MCS and Protein Phosphorescence
MCS and Protein Phosphorescence
Tryptophan phosphorescence within protein molecules is gaining attention as a probe of protein dynamics and structure. The tryptophan phosphorescence lifetime, τ, varies with the protein molecule’s local environment and conformation.

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